A. Kornberg (1992) has nicely discussed the DNA replication. In E.coli DNA replication has been investigated most extensively. It is thought that in eukaryotes probably similar mechanism operates. However, it has been found that in E.coli replication always starts at a very unique site called the origin. In E.coli the replicating apparatus contains an enzyme complex at the point where DNA thread is attached to plasmid membrane. Through this replicating point DNA thread moves and replication is accomplished. In eukaryotes enzyme moves along the DNA thread. It has earlier been described that E.coli possesses three types of DNA polymerases, each reads DNA template in 3’-->5’direction and catalyses the synthesis of DNA in 5’-->3’direction. The polymerases read deoxyribonucleotide triphoshates (dATP , dGRP, dTTP) as substrate and a DNA template. To the 3’ end of growing point, the nucleotides are added after interaction of 3’-OH end of dexyribose with alpha (first) phosphate group of substrate releasing pyrophosphate.
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Blogging is the new poetry. I find it wonderful and amazing in many ways.
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